Adrenomedullin and Adrenomedullin Binding Protein-1 Protect Endothelium-Dependent Vascular Relaxation in Sepsis

Adrenomedullin binding protein-1 modulates vascular responsiveness to adrenomedullin in late sepsis.

Adrenomedullin (AM), a potent vasodilatory peptide, plays an important role in initiating the hyperdynamic response during the early stage of sepsis. Moreover, the reduced vascular responsiveness to AM appears to be responsible for the transition from the early, hyperdynamic to the late, hypodynamic phase of sepsis. Although the novel specific AM binding protein-1 (AMBP-1) enhances AM-mediated ...

Adrenomedullin Binding Protein-1 Modulates Vascular Responsiveness to Adrenomedullin During the Late Stage of Sepsis

Adrenomedullin-induced endothelium-dependent relaxation in porcine ciliary arteries.

PURPOSE To investigate adrenomedullin-induced relaxation in isolated porcine ciliary arteries. METHODS In a myograph system (isometric force measurement), precontracted vessels ( approximately 0.1 micro M U46619; thromboxane A(2) analogue or approximately 10 nM endothelin-1) were exposed, in a cumulative manner, to increasing concentrations of adrenomedullin (1 nM to 1 micro M) in the presenc...

Modulation of cytokine responses by adrenomedullin and adrenomedullin binding protein-1 in macrophages: a novel pathway in sepsis.

Polymicrobial sepsis is a life-threatening disorder. Patients die of septic shock and multiple organ failure caused by lipopolysaccharide (LPS) and other bacterial products. A hyperdynamic phase is followed by shock and circulatory collapse. Myocardial dysfunction frequently accompanies severe sepsis and septic shock secondary to circulating depressant factors, including tumour necrosis factor-...

Circulating Precursor Levels of Endothelin-1 and Adrenomedullin, Two Endothelium-Derived, Counteracting Substances, in Sepsis

Plasma levels of endothelin-1 (ET-1) and adrenomedullin (ADM), two opposingly acting peptides, correlate with mortality in endotoxemia, but their measurement is cumbersome. New sandwich assays have been introduced that measure more stable precursor fragments. The objective of this study was to investigate the counterplay of their precursor peptides in septic patients and to compare them with di...